Journal article
Physiological characterisation of Penicillium chrysogenum strains expressing the expandase gene from Streptomyces clavuligerus during batch cultivations. Growth and adipoyl-7- aminodeacetoxycephalosporanic acid production
The production of adipoyl-7-aminodeacetoxy-cephalosporanic acid (ad-7-ADCA) was studied, using two recombinant strains of Penicillium chrysogenum carrying the expandase gene from Streptomyces clavuligerus. The adipoyl-side chain of this compound may easily be removed using an amidase; and this process therefore represents a new route for the production of 7-ADCA, which serves as a precursor for the production of many semi-synthetic cephalosporins.
In this study, one low- and one high-yielding strains were characterised and the specific productivities of ad-7-ADCA and byproducts of the biosynthetic pathway were compared. The fluxes through the biosynthetic pathway were quantified and it was found that there was a 30% higher flux through the expandase in the high-yielding strain.
In both strains, there was a significant degradation of adipate. Furthermore, the initial adipate concentration in batch cultures was shown to have a positive effect on the formation of ad-7-ADCA.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2001 |
Pages: | 357-362 |
ISSN: | 14320614 and 01757598 |
Types: | Journal article |
DOI: | 10.1007/s002530100787 |
Acid Production Adipates Biosynthetic Pathway Cephalosporin Cephalosporins Fructose Gluconates Glucose Intramolecular Transferases Legacy Mutagenesis, Insertional Penicillanic Acid Penicillin-Binding Proteins Penicillium chrysogenum Specific Productivity Streptomyces Sucrose adipic acid adipoyl-7-amino-3-deacetoxycephalosporanic acid aminopenicillanic acid deacetoxycephalosporin C synthetase gluconic acid