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Journal article

Quantification of specific E. coli in gut mucosa from Crohn's disease patients

By Jensen, Stina Rikke1,2; Fink, Lisbeth Nielsen1,2; Struve, Carsten3; Sternberg, Claus2,4; Andersen, Jens Bo5,6; Brynskov, Jørn7; Nielsen, Ole Haagen7; Pedersen, Susanne Brix1,2

From

Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark1

Department of Systems Biology, Technical University of Denmark2

Statens Serum Institut3

Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark4

Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark5

National Food Institute, Technical University of Denmark6

Copenhagen University Hospital Herlev and Gentofte7

We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.

Language: English
Year: 2011
Pages: 111-114
ISSN: 18728359 and 01677012
Types: Journal article
DOI: 10.1016/j.mimet.2011.04.002
ORCIDs: Sternberg, Claus , Pedersen, Susanne Brix , 0000-0003-1720-1581 and 0000-0003-4612-8635
Keywords

Adherent-invasive E. coli Bacterial quantification Crohn Disease Crohn's disease DNA Primers Escherichia coli Evaluation Studies Humans Intestinal Mucosa Journal Article Polymerase Chain Reaction Research Support, Non-U.S. Gov't qRT-PCR

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