Journal article
Key Players in the Genetic Switch of Bacteriophage TP901-1
After infection of a sensitive host temperate phages may enter either a lytic or a lysogenic pathway leading to new phage assembly or silencing as a prophage, respectively. The decision about which pathway to enter is centered in the genetic switch of the phage. In this work, we explore the bistable genetic switch of bacteriophage TP901-1 through experiments and statistical mechanical modeling.
We examine the activity of the lysogenic promoter Pr at different concentrations of the phage repressor, CI, and compare the effect of CI on Pr in the presence or absence of the phage-encoded MOR protein expressed from the lytic promoter Pl. We find that the presence of large amounts of MOR prevents repression of the Pr promoter, verifying that MOR works as an antirepressor.
We compare our experimental data with simulations based on previous mathematical formulations of this switch. Good agreement between data and simulations verify the model of CI repression of Pr. By including MOR in the simulations, we are able to discard a model that assumes that CI and MOR do not interact before binding together at the DNA to repress Pr.
The second model of Pr repression assumes the formation of a CI:MOR complex in the cytoplasm. We suggest that a CI:MOR complex may exist in different forms that either prevent or invoke Pr repression, introducing a new twist on mixed feedback systems.
Language: | English |
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Publisher: | The Biophysical Society |
Year: | 2011 |
Pages: | 313-321 |
ISSN: | 15420086 , 00063495 and 05236800 |
Types: | Journal article |
DOI: | 10.1016/j.bpj.2010.12.3681 |
ORCIDs: | 0000-0001-9820-3567 |
Amino Acid Sequence DNA-Binding Proteins Gene Expression Regulation, Viral Lysogeny Models, Genetic Molecular Dynamics Simulation Molecular Sequence Data ORF1 protein, Lactococcus lactis bacteriophage TP901-1 Plasmids Promoter Regions, Genetic Protein Binding Repressor Proteins Transcription, Genetic Viral Proteins Virus Activation repressor protein C1, bacteriophage