Journal article
Biomimetic triblock copolymer membrane arrays: a stable template for functional membrane proteins
It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants.
The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.
Language: | English |
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Year: | 2009 |
Pages: | 10447-10450 |
ISSN: | 07437463 and 15205827 |
Types: | Journal article |
DOI: | 10.1021/la902417m |
ORCIDs: | Helix Nielsen, Claus , 0000-0001-7543-9016 and 0000-0002-4258-8960 |