Journal article
Reactor design for minimizing product inhibition during enzymatic lignocellulose hydrolysis II. Quantification of inhibition and suitability of membrane reactors : II. Quantification of inhibition and suitability of membrane reactors
Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark2
CHEC Research Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark3
Product inhibition of cellulolytic enzymes affects the efficiency of the biocatalytic conversion of lignocellulosic biomass to ethanol and other valuable products. New strategies that focus on reactor designs encompassing product removal, notably glucose removal, during enzymatic cellulose conversion are required for alleviation of glucose product inhibition.
Supported by numerous calculations this review assesses the quantitative aspects of glucose product inhibition on enzyme-catalyzed cellulose degradation rates. The significance of glucose product inhibition on dimensioning of different ideal reactor types, i.e. batch, continuous stirred, and plug-flow, is illustrated quantitatively by modeling different extents of cellulose conversion at different reaction conditions.
The main operational challenges of membrane reactors for lignocellulose conversion are highlighted. Key membrane reactor features, including system set-up, dilution rate, glucose output profile, and the problem of cellobiose are examined to illustrate the quantitative significance of the glucose product inhibition and the total glucose concentration on the cellulolytic conversion rate.
Comprehensive overviews of the available literature data for glucose removal by membranes and for cellulose enzyme stability in membrane reactors are given. The treatise clearly shows that membrane reactors allowing continuous, complete, glucose removal during enzymatic cellulose hydrolysis, can provide for both higher cellulose hydrolysis rates and higher enzyme usage efficiency (kg(product/)kg(enzyme)).
Current membrane reactor designs are however not feasible for large scale operations. The report emphasizes that the industrial realization of cellulosic ethanol requires more focus on the operational feasibility within the different hydrolysis reactor designs, notably for membrane reactors, to achieve efficient enzyme-catalyzed cellulose degradation. (C) 2010 Elsevier Inc.
All rights reserved.
| Language: | English |
|---|---|
| Year: | 2010 |
| Pages: | 407-425 |
| ISSN: | 07349750 and 18731899 |
| Types: | Journal article |
| DOI: | 10.1016/j.biotechadv.2010.02.005 |
| ORCIDs: | Meyer, Anne S. , Jensen, Peter Arendt and Dam-Johansen, Kim |
Bacterial Physiological Phenomena Bioreactors Cellobiose Cellulases Computer Simulation Computer-Aided Design Enzyme Activation Enzyme Inhibitors Enzyme inhibition Equipment Design Glucose Glucose removal Hydrolysis Hydrolysis kinetics Lignin Membrane reactors Membranes, Artificial Models, Biological lignocellulose
