Journal article
A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis
We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N, N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels.
The performance of two different thiol-ene substrates (with 20% and 40% excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMAMAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption.
Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins.
Language: | English |
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Publisher: | Springer Vienna |
Year: | 2016 |
Pages: | 2111-2121 |
Journal subtitle: | Analytical Sciences Based on Micro- and Nanomaterials |
ISSN: | 14365073 , 00768642 and 00263672 |
Types: | Journal article |
DOI: | 10.1007/s00604-016-1825-4 |
ORCIDs: | 0000-0003-1065-1985 |