Journal article
Antibacterial compounds from marine Vibrionaceae isolated on a global expedition
Division of Seafood Research, National Food Institute, Technical University of Denmark1
National Food Institute, Technical University of Denmark2
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3
Department of Systems Biology, Technical University of Denmark4
Organic Chemistry, Department of Chemistry, Technical University of Denmark5
Department of Chemistry, Technical University of Denmark6
On a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. Three hundred of the antibacterial strains were assigned to the Vibrionaceae family. The purpose of the present study was to investigate the phylogeny and bioactivity of five Vibrionaceae strains with pronounced antibacterial activity.
These were identified as Vibrio coralliilyticus (two strains), V. neptunius (two strains), and Photobacterium halotolerans (one strain) on the basis of housekeeping gene sequences. The two related V. coralliilyticus and V. neptunius strains were isolated from distant oceanic regions. Chemotyping by LC-UV/MS underlined genetic relationships by showing highly similar metabolite profiles for each of the two V. coralliilyticus and V. neptunius strains, respectively, but a unique profile for P. halotolerans.
Bioassay-guided fractionation identified two known antibiotics as being responsible for the antibacterial activity; andrimid (from V. coralliilyticus) and holomycin (from P. halotolerans). Despite the isolation of already known antibiotics, our findings show that marine Vibrionaceae are a resource of antibacterial compounds and may have potential for future natural product discovery.
Language: | English |
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Publisher: | Molecular Diversity Preservation International |
Year: | 2010 |
Pages: | 2946-2960 |
ISSN: | 16603397 |
Types: | Journal article |
DOI: | 10.3390/md8122946 |
ORCIDs: | Gotfredsen, Charlotte Held , Larsen, Thomas Ostenfeld and Gram, Lone |
Anti-Bacterial Agents Antibiosis Biology (General) Drug Discovery Lactams Microbial Sensitivity Tests Molecular Structure Oceans and Seas Photobacterium Photobacterium halotolerans Phylogeny Polyenes Pyrroles QH301-705.5 RNA, Ribosomal, 16S Seawater Staphylococcus aureus Vibrio Vibrio coralliilyticus Vibrio neptunius Vibrionaceae andrimid chemotyping holomycin