Journal article
Investigation of diversity of plasmids carrying the blaTEM-52 gene
OBJECTIVES: To investigate the diversity of plasmids that carry blaTEM-52 genes among Escherichia coli and Salmonella enterica originating from animals, meat products and humans. METHODS: A collection of 22 blaTEM-52-encoding plasmids was characterized by restriction fragment length polymorphism (RFLP), replicon typing (by PCR or replicon sequencing), susceptibility testing, assessment of plasmid ability to self-transfer by conjugation and typing of the genetic environment of the blaTEM-52 gene.
Detected IncI1 plasmids underwent further plasmid multilocus sequence typing. RESULTS: RFLP profiles demonstrated dissemination of blaTEM-52 in Denmark (imported meat from Germany), France, Belgium and the Netherlands from 2000 to 2006 by mainly two different plasmids, one encoding blaTEM-52b (IncX1A, 45 kb) and the other blaTEM-52c (IncI1, 80 kb).
In addition, blaTEM-52b was also found to be located on various other plasmids belonging to IncA/C and IncL/M, while blaTEM-52c was found on IncN-like as well as on IncR plasmids. In the majority of cases (n = 21) the blaTEM-52 gene was located on a Tn3 transposon. Seven out of 10 blaTEM-52 plasmids tested in conjugation experiments were shown to be capable of self-transfer to a plasmid-free E. coli recipient.
CONCLUSIONS: The blaTEM-52 gene found in humans could have been transmitted on transferable plasmids originating from animal sources. Some of the blaTEM-52 plasmids carry replicons that differ from the classical ones. Two novel replicons were detected, IncX1A and IncN-like. Unlike its predecessor blaTEM-1, the blaTEM-52 gene was not detected on F-type replicons suggesting that this gene evolved on other types of plasmid scaffolds.
Language: | English |
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Publisher: | Oxford University Press |
Year: | 2011 |
Pages: | 2465-2474 |
ISSN: | 14602091 and 03057453 |
Types: | Journal article |
DOI: | 10.1093/jac/dkr331 |
ORCIDs: | 0000-0001-6227-9906 and 0000-0003-0027-1524 |
Animals DNA Transposable Elements DNA, Bacterial Drug Resistance, Multiple, Bacterial Escherichia coli Europe Gene Transfer, Horizontal Genetic Variation Humans Microbial Sensitivity Tests Multilocus Sequence Typing Plasmids Polymorphism, Restriction Fragment Length Salmonella enterica TEM-52 beta-lactamase Tn3 transposase Transposases antibiotic resistance beta-Lactam Resistance beta-Lactamases beta-Lactams human and non-human isolates