Journal article
The extent to which ATP demand controls the glycolytic flux depends strongly on the organism and conditions for growth
Using molecular genetics we have introduced uncoupled ATPase activity in two different bacterial species, Escherichia coli and Lactococcus lactis, and determined the elasticities of the growth rate and glycolytic flux towards the intracellular [ATP]/[ADP] ratio. During balanced growth in batch cultures of E. coli the ATP demand was found to have almost full control on the glycolytic flux (FCC=0.96) and the flux could be stimulated by 70%.
In contrast to this, in L. lactis the control by ATP demand on the glycolytic flux was close to zero. However, when we used non-growing cells of L. lactis (which have a low glycolytic flux) the ATP demand had a high flux control and the flux could be stimulated more than two fold. We suggest that the extent to which ATP demand controls the glycolytic flux depends on how much excess capacity of glycolysis is present in the cells.
| Language: | English |
|---|---|
| Publisher: | Kluwer Academic Publishers |
| Year: | 2002 |
| Pages: | 41-45 |
| Journal subtitle: | An International Journal on Molecular and Cellular Biology |
| ISSN: | 03014851 and 15734978 |
| Types: | Journal article |
| DOI: | 10.1023/a:1020398117281 |
| ORCIDs: | Solem, Christian and Jensen, Peter Ruhdal |
ATPase Adenosine Diphosphate Adenosine Triphosphatases Adenosine Triphosphate Animal Anatomy / Morphology / Histology Animal Biochemistry Bacterial Proteins Escherichia coli Glycolysis Lactococcus lactis Life Sciences MCA Protein Subunits coli glycolysis lactococcus metabolic control analysis
