Journal article
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans
Norwegian University of Life Sciences1
Department of Biotechnology and Biomedicine, Technical University of Denmark2
Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Protein Glycoscience and Biotechnology, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
University of Michigan, Ann Arbor5
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Regulatory Genomics, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark7
β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans.
We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment.
In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2019 |
Pages: | 905 |
ISSN: | 20411723 |
Types: | Journal article |
DOI: | 10.1038/s41467-019-08812-y |
ORCIDs: | 0000-0003-3527-8101 , Hansen, Morten Ejby , 0000-0002-2067-4059 , Hachem, Maher Abou , Leth, Maria Louise and Workman, Christopher T. |