Journal article
Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents
Pseudomonas aeruginosa is an opportunistic pathogen which is responsible for a wide range of infections. Production of virulence factors and biofilm formation by P. aeruginosa are partly regulated by cell-to-cell communication quorum-sensing systems. Identification of quorum-quenching reagents which block the quorum-sensing process can facilitate development of novel treatment strategies for P. aeruginosa infections.
We have used molecular dynamics simulation and experimental studies to elucidate the efficiencies of two potential quorum-quenching reagents, triclosan and green tea epigallocatechin gallate (EGCG), which both function as inhibitors of the enoyl-acyl carrier protein (ACP) reductase (ENR) from the bacterial type II fatty acid synthesis pathway.
Our studies suggest that EGCG has a higher binding affinity towards ENR of P. aeruginosa and is an efficient quorum-quenching reagent. EGCG treatment was further shown to be able to attenuate the production of virulence factors and biofilm formation of P. aeruginosa.
Language: | English |
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Publisher: | Molecular Diversity Preservation International |
Year: | 2010 |
Pages: | 780-792 |
ISSN: | 14315157 and 14203049 |
Types: | Journal article |
DOI: | 10.3390/molecules15020780 |
ORCIDs: | Sternberg, Claus and Molin, Søren |
Pseudomonas aeruginosa enoyl-acyl carrier protein reductase molecular dynamics simulation quorum-quenching
Amino Acid Sequence Biofilms Catechin Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) Enzyme Inhibitors Green Fluorescent Proteins Hydrogen Bonding Indicators and Reagents Molecular Dynamics Simulation Molecular Sequence Data Movement Organic chemistry QD241-441 Quorum Sensing Sequence Alignment Triclosan Virulence epigallocatechin gallate