Journal article
A Simplified Chromatographic Approach to Purify Commercially Available Bovine Submaxillary Mucins (BSM)
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark1
Department of Mechanical Engineering, Technical University of Denmark2
Materials and Surface Engineering, Department of Mechanical Engineering, Technical University of Denmark3
University of Southern Denmark4
Department of Systems Biology, Technical University of Denmark5
In this study, a simple purification protocol is developed to reduce the bovine serum albumin (BSA) content in commercially available bovine submaxillary mucin (BSM). This involved purification of the BSM by one-column anion-exchange chromatography protocol resulting in BSM with greatly reduced BSA content and homogeneously distributed size, and in a high yield of 43% from BSM as received from the manufacturer.
The purity and composition of commercially acquired BSM were assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry, which verified that BSA is the most abundant nonmucinous protein component. The purification effect was evident from a significantly altered circular dichroism (CD) spectrum of BSM after anion-exchange chromatography.
| Language: | English |
|---|---|
| Publisher: | Taylor & Francis Group |
| Year: | 2015 |
| Pages: | 84-99 |
| ISSN: | 15322297 and 10826068 |
| Types: | Journal article |
| DOI: | 10.1080/10826068.2014.887583 |
| ORCIDs: | Svensson, Birte , Abou Hachem, Maher and Lee, Seunghwan |
Anion-exchange chromatography (AEC) Bovine serum albumin (BSA) Bovine submaxillary mucin (BSM) Mucin Purification
Animals BSM1 protein, Bos taurus Cattle Chromatography, Ion Exchange Chromatography, Thin Layer Circular Dichroism Electrophoresis, Polyacrylamide Gel Mucins Serum Albumin, Bovine Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization anion-exchange chromatography (AEC) bovine serum albumin (BSA) bovine submaxillary mucin (BSM) mucin purification
