Journal article
Validation of QuEChERS for screening of 4 marker polycyclic aromatic hydrocarbons in fish and malt
Application of QuEChERS as a new quick and easy method for screening of polycyclic aromatic hydrocarbons (PAHs) was developed, validated and used for quantification (GC-QTOF-MS) of 4 PAHs, namely benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene. A clean-up step using Zirconium based dispersive SPE was applied.
Validation based on spiked samples (0.5, 1 and 4 μg/kg) revealed a limit of quantification (LOQ) for the 4 PAH compounds in the range of 0.14–0.24 μg/kg for fish and 0.12–0.24 μg/kg for raw barley. Overall recoveries ranged from 88 to 117% with repeatability and in-house reproducibility from 2.6 to 16%.
The method was applied to 9 malt samples which were found to contain the sum of 4 PAH from <0.36 μg/kg for raw barley to 26 μg/kg for peat smoked barley. For 5 smoked fish samples the sum of PAH4 ranged from <0.34 μg/kg for cold smoked salmon to 2.2 μg/kg for hot smoked mackerel. In general it was found that the method could be used as a fast screening for evaluation of smoked fish according to EU Commission maximum concentrations of benzo[a]pyrene (2.0 μg/kg) and the sum of PAH4 (10 μg/kg).
Language: | English |
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Year: | 2020 |
Pages: | 106434 |
ISSN: | 09567135 and 18737129 |
Types: | Journal article |
DOI: | 10.1016/j.foodcont.2018.12.010 |
ORCIDs: | Duedahl-Olesen, Lene and Krüger Jensen, Lisbeth |