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Journal article

Restriction Fragment Pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus)

From

Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark1

National Food Institute, Technical University of Denmark2

Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool.

However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships.

A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.

Language: English
Publisher: Springer-Verlag
Year: 1987
Pages: 215-224
ISSN: 14328798 and 03048608
Types: Journal article
DOI: 10.1007/BF01314422

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