Journal article
System-level perturbations of cell metabolism using CRISPR/Cas9
CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modifications and transcriptional regulations.
The effectiveness of these tools allows researchers to implement genome-wide perturbations, test model-guided genome editing strategies, and perform transcriptional reprogramming perturbations in a more advanced manner than previously possible. In this mini-review we highlight recent studies adopting CRISPR/Cas9 for systems-level perturbations and model-guided metabolic engineering.
Language: | English |
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Year: | 2017 |
Pages: | 134-140 |
ISSN: | 18790429 and 09581669 |
Types: | Journal article |
DOI: | 10.1016/j.copbio.2017.03.014 |
ORCIDs: | Jakociunas, Tadas and Jensen, Michael Krogh |