Journal article
Signifiance of Arginine 20 in the 2A protease for swine vesicular disease virus pathogenicity
National Agriculture and Food Research Organization1
Pirbright Institute2
Section of Vesicular virus diseases, Division of Virology, National Veterinary Institute, Technical University of Denmark3
Division of Virology, National Veterinary Institute, Technical University of Denmark4
National Veterinary Institute, Technical University of Denmark5
Pathogenic and attenuated strains of swine vesicular disease virus (SVDV), an enterovirus, have been characterized previously and, by using chimeric infectious cDNA clones, the key determinants of pathogenicity in pigs have been mapped to the coding region for 1Dā2A. Within this region, residue 20 of the 2A protease is particularly significant.
Inoculation of pigs with mutant viruses containing single amino acid substitutions at this residue leads to the appearance of revertants, often containing an arginine at this position encoded by an AGA codon, one of six codons for this residue. The properties in pigs of two chimeric viruses, each with an arginine residue at this position but encoded by different codons, have been investigated in parallel with the parental pathogenic and attenuated strains.
Presence of the arginine residue, but not of the AGA codon, is essential for induction of high viraemia and appearance of significant disease.
Language: | English |
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Year: | 2007 |
Pages: | 2275-2279 |
ISSN: | 14652099 and 00221317 |
Types: | Journal article |
DOI: | 10.1099/vir.0.82920-0 |
ORCIDs: | Belsham, Graham |