Journal article
AU Content in the MicroRNA Sequence Influences its Stability After Heat Treatment
Immunobiology and Biomimetics, Department of Health Technology, Technical University of Denmark1
Department of Health Technology, Technical University of Denmark2
Mucosal Immunology, Division of Immunology & Vaccinology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Innate Immunology, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Department of Biotechnology and Biomedicine, Technical University of Denmark6
Innate Immunology, Division of Immunology & Vaccinology, National Veterinary Institute, Technical University of Denmark7
University of Copenhagen8
Tranlational Immunology, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark9
BACKGROUND: MicroRNAs (miRNAs) are short non-coding RNA molecules which regulate gene expression post-transcriptionally and are involved in a multitude of cellular processes. MiRNAs are known to be very stable compared to messenger RNAs (mRNAs), making them excellent candidates as biomarkers for disease.
Recently, studies have suggested that miRNA stability in formalin fixed samples might dependend on their nucleotide composition. OBJECTIVE: To explore the stability of a panel of miRNAs isolated from porcine blood and lung tissue after heat and enzyme treatment. METHOD: Porcine RNA isolated from lung tissue and blood leukocytes were used for this study.
RNA samples were exposed to heat treatment and RNAse A digestion. The levels of selected miRNAs were measured by means of qPCR before and after heat and enzyme treatment. RESULTS: Fourteen miRNAs were successfully analysed, and they were found to degrade at differently after exposure to heat or RNAse A.
MiRNAs with <60% of adenine (A) and uracil (U)in their sequence were found to be more stable. CONCLUSION: This is the first study showing that different miRNAs isolated from lung tissue display unequal stability after heat treatment, probably based on their nucleotide composition, highlighting the importance of considering the miRNA sequence when investigating their value as biomarkers.
| Language: | English |
|---|---|
| Year: | 2019 |
| Pages: | 216-222 |
| ISSN: | 22115374 and 22115366 |
| Types: | Journal article |
| DOI: | 10.2174/2211536608666190131102252 |
| ORCIDs: | Brogaard, Louise , Heegaard, Peter M. H. and Skovgaard, Kerstin |
% AU Heat treatment RNAse A digestion Saliva Stability miRNA
