Journal article
Nonbioluminescent strains of Photobacterium phosphoreum produce the cell-to-cell communication signal N-(3-Hydroxyoctanoyl)homoserine lactone
National Institute of Aquatic Resources, Technical University of Denmark1
Section for Aquatic Microbiology and Seafood Hygiene, National Institute of Aquatic Resources, Technical University of Denmark2
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark3
National Food Institute, Technical University of Denmark4
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark5
Department of Systems Biology, Technical University of Denmark6
Center for Biomedical Microbiology, Department of Systems Biology, Technical University of Denmark7
Bioluminescence is a common phenotype in marine bacteria, such As Vibrio and Photobacterium species, and can be quorum regulated by N-acylated homoserine lactones (AHLs). We extracted a molecule that induced a bacterial AHL monitor (Agrobacterium tumefaciens NT1 [pZLR4]) from packed cod fillets, which spoil due to growth of Photobacterium phosphoreum.
Interestingly, AHLs were produced by 13 nonbioluminescent strains of P. phosphoreum isolated from the product. Of 177 strains of P. phosphoreum (including 18 isolates from this study), none of 74 bioluminescent strains elicited a reaction in the AHL monitor, whereas 48 of 103 nonbioluminescent strains did produce AHLs.
AHLs were also detected in Aeromonas spp., but not in Shewanella strains. Thin-layer chromatographic profiles of cod extracts and P. phosphoreum culture supernatants identified a molecule similar in relative mobility (R-f value) and shape to N-(3-hydroxyoctanoyl)homoserine lactone, and the presence of this molecule in culture supernatants from a nonbioluminescent strain of P. phosphoreum was confirmed by high-performance liquid chromatography-positive electrospray high-resolution mass spectrometry.
Bioluminescence (in a non-AHL-producing strain of P. phosphoreum) was strongly up-regulated during growth, whereas AHL production in a nonbioluminescent strain of P. phosphoreum appeared constitutive. AHLs apparently did not influence bioluminescence, as the addition of neither synthetic AHLs nor supernatants delayed or reduced this phenotype in luminescent strains of P. phosphoreum.
The phenotypes of nonbioluminescent P. phosphoreum strains regulated by AHLs remains to be elucidated.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2005 |
Pages: | 2113-2120 |
ISSN: | 10985336 and 00992240 |
Types: | Journal article |
DOI: | 10.1128/AEM.71.4.2113-2120.2005 |
ORCIDs: | Dalgaard, Paw , Nielsen, Kristian Fog and Gram, Lone |
4-Butyrolactone Animals Chromatography, High Pressure Liquid Chromatography, Thin Layer Culture Media Food Packaging Gadus morhua Gene Expression Regulation, Bacterial Luminescence Mass Spectrometry Molecular Sequence Data Photobacterium RNA, Ribosomal, 16S Sequence Analysis, DNA Signal Transduction homoserine lactone