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Journal article

Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

From

Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark1

Department of Systems Biology, Technical University of Denmark2

H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.

Language: English
Publisher: American Society for Microbiology
Year: 2001
Pages: 6707-6709
ISSN: 00219193 , 10985530 and 10678832
Types: Journal article
DOI: 10.1128/JB.183.22.6707-6709.2001
ORCIDs: Jensen, Peter Ruhdal
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