Journal article
Influence of the adipate and dissolved oxygen concentrations on the beta-lactam production during continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus
The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from I to 37.5 g l(-1) and from 2% to 125% air saturation (%AS), respectively.
The total beta-lactams specific productivity, r(p) (total) was not significantly changed for adipate concentrations from 5 to 25 g l(-1), but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r(p) (total) was stable around 18 mumol g DW-1 h(-1) for DOC being in the range from 15 to 125%AS.
When DOC was decreased from 15 to 7%AS, r(p) (total) increased to 25 mumol g DW-1 h(-1), mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.
| Language: | English |
|---|---|
| Year: | 2003 |
| Pages: | 42-48 |
| ISSN: | 10967184 and 10967176 |
| Types: | Journal article |
| DOI: | 10.1016/s1096-7176(03)00006-5 |
Adipates Bioreactors Cell Culture Techniques Cephalosporins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Oxygen Oxygen Consumption Penicillium chrysogenum Protein Engineering Recombinant Proteins Solubility Species Specificity Streptomyces adipic acid adipoyl-7-amino-3-deacetoxycephalosporanic acid beta-Lactams
