Journal article
Predictable tuning of protein expression in bacteria
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Bacterial Synthetic Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Bacterial Cell Factory Optimization, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
iLoop, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases.
Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2016 |
| Pages: | 233-236 |
| Journal subtitle: | Techniques for Life Scientists and Chemists |
| ISSN: | 15487105 and 15487091 |
| Types: | Journal article |
| DOI: | 10.1038/nmeth.3727 |
| ORCIDs: | Klausen, Michael Schantz , Ingemann Jensen, Sheila , Wulff, Tune , Nielsen, Alex Toftgaard , Herrgard, Markus and Sommer, Morten Otto Alexander |
