Journal article
Phylogenetic analysis of Monascus and new species from honey, pollen and nests of stingless bees
Universidade Federal de Pernambuco1
Swedish University of Agricultural Sciences2
Westerdijk Fungal Biodiversity Institute3
Department of Biotechnology and Biomedicine, Technical University of Denmark4
Fungal Chemodiversity, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
The genus Monascus was described by van Tieghem (1884) to accommodate M. ruber and M. mucoroides, two species with non-ostiolate ascomata. Species delimitation in the genus is still mainly based on phenotypic characters, and taxonomic studies that include sequence data are limited. The genus is of economic importance.
Species are used in fermented Asian foods as food colourants (e.g. 'red rice' (ang-kak, angka)) and found as spoilage organisms, and recently Monascus was found to be essential in the lifecycle of stingless bees. In this study, a polyphasic approach was applied combining morphological characters, ITS, LSU, β-tubulin, calmodulin and RNA polymerase II second largest subunit sequences and extrolite data, to delimit species and to study phylogenetic relationships in Monascus.
Furthermore, 30 Monascus isolates from honey, pollen and nests of stingless bees in Brazil were included. Based on this polyphasic approach, the genus Monascus is resolved in nine species, including three new species associated with stingless bees (M. flavipigmentosus sp. nov., M. mellicola sp. nov., M. recifensis sp. nov., M. argentinensis, M. floridanus, M. lunisporas, M. pallens, M. purpureus, M. ruber), and split in two new sections (section Floridani sect. nov., section Rubri sect. nov.).
Phylogenetic analysis showed that the xerophile Monascus eremophilus does not belong in Monascus and monophyly in Monascus is restored with the transfer of M. eremophilus to Penicillium (P. eremophilum comb. nov.). A list of accepted and excluded Monascus and Basipetospora species is given, together with information on (ex-)types cultures and barcode sequence data.
Language: | English |
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Publisher: | CBS Fungal Biodiversity Centre |
Year: | 2017 |
Pages: | 29-51 |
ISSN: | 18729797 and 01660616 |
Types: | Journal article |
DOI: | 10.1016/j.simyco.2017.04.001 |
ORCIDs: | Frisvad, Jens Christian |
Biology (General) Monascus flavipigmentosus R.N. Barbosa, Souza-Motta, N.T. Oliveira & Houbraken Monascus mellicola R.N. Barbosa, Souza-Motta, N.T. Oliveira & Houbraken Monascus recifensis R.N. Barbosa, Souza-Motta, N.T. Oliveira & Houbraken Monascus section Floridani R.N. Barbosa & Houbraken Monascus section Rubri R.N. Barbosa & Houbraken Penicillium eremophilum (A.D. Hocking & Pitt) Houbraken, Leong & Vinnere-Pettersson QH301-705.5