Journal article
A device for extraction, manipulation and stretching of DNA from single human chromosomes
NSE-Optofluidics Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Nano-Bio Integrated Systems Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
University of Oxford6
We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit.
A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.
| Language: | English |
|---|---|
| Publisher: | The Royal Society of Chemistry |
| Year: | 2011 |
| Pages: | 1431-1433 |
| ISSN: | 14730189 and 14730197 |
| Types: | Journal article |
| DOI: | 10.1039/c0lc00603c |
| ORCIDs: | Rasmussen, Kristian Hagsted , Marie, Rodolphe , Svendsen, Winnie Edith and Kristensen, Anders |
