Journal article
Characterization of a patch-clamp microchannel array towards neuronal networks analysis
ChemLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Fluidic Array Systems and Technology Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
The attempt to combine the planar patch clamping idea with the microelectrode array (MEA) concept has led to the fabrication of a patch clamp micro-channel array (PC mu CA). Such a system is thought to be a powerful framework for neuroscience research and drug screening, as a novel tool for simultaneous patch clamping of cultured cells or neurons in the same network.
A disposable silicon/silicon dioxide (Si/SiO2) chip with a microhole array was integrated in a microfluidic system for cell handling, perfusion and electrical recording. Fluidic characterization showed that our PC mu CA can work as a precise local perfusion system for chemicals or drugs. Electrical characterization for microholes of 2 mu m and 3 mu m revealed an access resistance of 8.09 +/- 0.84 M Omega and 3.18 +/- 0.63 M Omega, respectively.
The capacitance was 98.6 +/- 13.2 pF. The values are close to what can be expected from theory, but the capacitance is still too high for high resolution recording. The system was tested on HeLa cells: successful cell trapping with a sealing of 40 M Omega was recorded. Modification of the Si/SiO2 chip is needed in order to achieve a better sealing and long-term cell culturing in the PC mu CA remains to be tested.
Language: | English |
---|---|
Publisher: | Springer-Verlag |
Year: | 2010 |
Pages: | 963-972 |
ISSN: | 16134990 and 16134982 |
Types: | Journal article |
DOI: | 10.1007/s10404-010-0619-y |
ORCIDs: | Dufva, Martin |