Journal article
No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs
National Veterinary Institute, Technical University of Denmark1
Diagnostic & Development, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark2
Virology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark3
Danish Agriculture and Food Council4
Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark5
Bacteriology & Parasitology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark6
Swedish University of Agricultural Sciences7
Statens Serum Institut8
Section for Virology, National Veterinary Institute, Technical University of Denmark9
University of Copenhagen10
...and 0 moreThe aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis.
Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods. A total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively.
The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2).
Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each.
Our detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.
Language: | English |
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Publisher: | BioMed Central |
Year: | 2017 |
Pages: | 315 |
Journal subtitle: | Bmc Series – Open, Inclusive and Trusted |
ISSN: | 17466148 |
Types: | Journal article |
DOI: | 10.1186/s12917-017-1239-5 |
ORCIDs: | Goecke, Nicole Bakkegård , Hjulsager, Charlotte Kristiane , Boye, Mette , Larsen, L. E. and 0000-0001-6811-5881 |