Journal article
64Cu loaded liposomes as positron emission tomography imaging agents
Colloids and Biological Interfaces Group, Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
University of Copenhagen4
Biomedical Tracers, Radiation Research Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark5
Radiation Research Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark6
Risø National Laboratory for Sustainable Energy, Technical University of Denmark7
We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide (64Cu) using a new ionophore, 2-hydroxyquinoline, to carry 64Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator.
Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the 64Cu-liposomes highly applicable as PET imaging agents. We show the utility of the 64Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.
Language: | English |
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Year: | 2011 |
Pages: | 2334-2341 |
ISSN: | 18785905 and 01429612 |
Types: | Journal article |
DOI: | 10.1016/j.biomaterials.2010.11.059 |
ORCIDs: | 0000-0002-2706-5547 , Henriksen, Jonas Rosager , Elema, Dennis Ringkjøbing and Andresen, Thomas Lars |