Journal article
Dielectrophoresis microsystem with integrated flow cytometers for on-line monitoring of sorting efficiency
Department of Micro- and Nanotechnology, Technical University of Denmark1
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
BioLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Center for Individual Nanoparticle Functionality, Centers, Technical University of Denmark5
Center for Nanoteknologi, Centers, Technical University of Denmark6
Silicon Microtechnology Group, MicroElectroMechanical Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark7
MicroElectroMechanical Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark8
Risø National Laboratory for Sustainable Energy, Technical University of Denmark9
Surface Engineering Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark10
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark11
ChemLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark12
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark13
Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark14
...and 4 moreDielectrophoresis (DEP) and flow cytometry are powerful technologies and widely applied in microfluidic systems for handling and measuring cells and particles. Here, we present a novel microchip with a DEP selective filter integrated with two microchip flow cytometers (FCs) for on-line monitoring of cell sorting processes.
On the microchip, the DEP filter is integrated in a microfluidic channel network to sort yeast cells by positive DER The two FCs detection windows are set upstream and downstream of the DEP filter. When a cell passes through the detection windows, the light scattered by the cell is measured by integrated polymer optical elements (waveguide, lens, and fiber coupler).
By comparing the cell counting rates measured by the two FCs, the collection efficiency of the DEP filter can be determined. The chips were used for quantitative determination of the effect of flow rate, applied voltage, conductivity of the sample, and frequency of the electric field on the sorting efficiency.
A theoretical model for the capture efficiency was developed and a reasonable agreement with the experimental results observed. Viable and non-viable yeast cells showed different frequency dependencies and were sorted with high efficiency. At 2 MHz, more than 90% of the viable and less than 10% of the non-viable cells were captured on the DEP filter.
The presented approach provides quantitative real-time data for sorting a large number of cells and will allow optimization of the conditions for, e.g., collecting cancer cells on a DEP filter while normal cells pass through the system. Furthermore, the microstructure is simple to fabricate and can easily be integrated with other microstructures for labon-a-chip applications.
Language: | English |
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Publisher: | WILEY-VCH Verlag |
Year: | 2006 |
Pages: | 5081-5092 |
ISSN: | 15222683 and 01730835 |
Types: | Journal article |
DOI: | 10.1002/elps.200600422 |
ORCIDs: | Hansen, Ole , Bang, Dang Duong and Wolff, Anders |