Journal article
Chronic rejection of a lung transplant is characterized by a profile of specific autoantibodies : Autoantibody profiling of chronic rejection
Risø National Laboratory for Sustainable Energy, Technical University of Denmark1
Copenhagen University Hospital Herlev and Gentofte2
ImmunArray, Ltd.3
Weizmann Institute of Science4
Stochastic Systems and Signals Group, Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
Department of Micro- and Nanotechnology, Technical University of Denmark7
Obliterative bronchiolitis (OB) continues to be the major limitation to long-term survival after lung transplantation. The specific aetiology and pathogenesis of OB are not well understood. To explore the role of autoreactivity in OB, we spotted 751 different self molecules onto glass slides, and used these antigen microarrays to profile 48 human serum samples for immunoglobulin G (IgG) and IgM autoantibodies; 27 patients showed no or mild bronchiolitis obliterans syndrome (BOS; a clinical correlate of OB) and 15 patients showed medium to severe BOS.
We now report that these BOS grades could be differentiated by a profile of autoantibodies binding to 28 proteins or their peptides. The informative autoantibody profile included down-regulation as well as up-regulation of both IgM and IgG specific reactivities. This profile was evaluated for robustness using a panel of six independent test patients.
Analysis of the functions of the 28 informative self antigens showed that eight of them are connected in an interaction network involved in apoptosis and protein metabolism. Thus, a profile of autoantibodies may reflect pathological processes in the lung allograft, suggesting a role for autoimmunity in chronic rejection leading to OB.
Language: | English |
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Publisher: | Blackwell Science Inc |
Year: | 2010 |
Pages: | 427-435 |
ISSN: | 13652567 , 00192805 and 09534954 |
Types: | Journal article |
DOI: | 10.1111/j.1365-2567.2010.03246.x |
ORCIDs: | Flyvbjerg, Henrik |
antigen microarray apoptosis autoantibodies bronchiolitis obliterans syndrome lung transplantation uden tema
Adult Apoptosis Autoantibodies Autoantigens Blood Pressure Bronchiolitis Obliterans Chemotaxis Disease Progression Female Graft Rejection Humans Immunoglobulin G Immunoglobulin M Lung Transplantation Male Metabolic Networks and Pathways Microarray Analysis Middle Aged Principal Component Analysis Proteins