Journal article
Biodegradable Polymeric Substances Produced by a Marine Bacterium from a Surplus Stream of the Biodiesel Industry
CSIR - Central Salt Marine Chemicals Research Institute1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Bacterial Signal Transduction, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Algallio Biotech Private Limited5
Crude glycerol is generated as a by-product during transesterification process and during hydrolysis of fat in the soap-manufacturing process, and poses a problem for waste management. In the present approach, an efficient process was designed for simultaneous production of 0.2 g/L extracellular epsilon-polylysine and 64.6% (w/w) intracellular polyhydroxyalkanoate (PHA) in the same fermentation broth (1 L shake flask) utilizing Jatropha biodiesel waste residues as carbon rich source by marine bacterial strain (Bacillus licheniformis PL26), isolated from west coast of India.
The synthesized epsilon-polylysine and polyhydroxyalkanoate PHA by Bacillus licheniformis PL26 was characterized by thermogravimetric analysis (TGA), differential scanning colorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and H-1 Nuclear magnetic resonance spectroscopy (NMR). The PHA produced by Bacillus licheniformis was found to be poly-3-hydroxybutyrate-co-3-hydroxyvalerate (P3HB-co-3HV).
The developed process needs to be statistically optimized further for gaining still better yield of both the products in an efficient manner.
Language: | English |
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Publisher: | MDPI |
Year: | 2016 |
Pages: | 34 |
ISSN: | 23065354 |
Types: | Journal article |
DOI: | 10.3390/bioengineering3040034 |
ORCIDs: | Singh, Priyanka |