Journal article
TOL plasmid carriage enhances biofilm formation and increases extracellular DNA content in Pseudomonas putida KT2440 : TOL plasmid enhances P. putida biofims
Adherent growth of Pseudomonas putida KT2440 with and without the TOL plasmid (pWWO) at the solid-liquid and air-liquid interface was examined. We compared biofilm formation on glass in flow cells, and assayed pellicle (air-liquid interface biofilm) formation in stagnant liquid cultures by confocal laser scanning microscopy.
The TOL-carrying strains formed pellicles and thick biofilms, whereas the same strains without the plasmid displayed little adherent growth. Microscopy using fluorescent nucleic acid-specific stains revealed differences in the production of extracellular polymeric substances: TOL carriage leads to more extracellular DNA (eDNA) in pellicles and biofilms.
Pellicles were dissolved by DNase I treatment. Enhanced cell lysis due to plasmid carriage was ruled out as the mechanism for eDNA release. We report, for the first time, that carriage of a conjugative plasmid leads to increased biofilm formation by production of eDNA.
Language: | English |
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Year: | 2010 |
Pages: | 84-92 |
ISSN: | 15746968 and 03781097 |
Types: | Journal article |
DOI: | 10.1111/j.1574-6968.2010.02105.x |
ORCIDs: | Smets, Barth F. |
BRIC Biofilms DNA, Bacterial Extracellular Space Plasmids Pseudomonas putida