Journal article
Determination of 5 '-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs
Technical University of Denmark1
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark2
Division of Virology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Stald/vægterservice, Division of Virology, National Veterinary Institute, Technical University of Denmark5
We determined the untranslated 5'-leader sequence for three different isolates of porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- and American-types, as well as an American-type vaccine strain. 5'-leader from European- and American-type PRRSV differed in length (220 and 190 nt, respectively), and exhibited only approximately 50% nucleotide homology.
Nevertheless, highly conserved areas were identified in the leader of all 3 PRRSV isolates, which constitute candidate motifs for binding of protein(s) involved in viral replication. These comparative data provide a priori knowledge for mutational identification of virulence determinants in the 5' nontranslated part of the PRRSV genome.
| Language: | English |
|---|---|
| Publisher: | Springer-Verlag |
| Year: | 1999 |
| Pages: | 981-987 |
| ISSN: | 14328798 and 03048608 |
| Types: | Journal article |
| DOI: | 10.1007/s007050050560 |
5' Untranslated Regions Animals Base Sequence Biomedicine Conserved Sequence Europe Infectious Diseases Medical Microbiology Molecular Sequence Data North America Porcine respiratory and reproductive syndrome virus RNA, Messenger RNA, Viral Sequence Alignment Sequence Homology, Nucleic Acid Swine Virology Virus Replication
