Journal article
Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001
Technical University of Denmark1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Natural Product Discovery, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
DTU Metabolomics Core, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark5
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Bacterial Interactions and Evolution, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark7
Department of Biotechnology and Biomedicine, Technical University of Denmark8
University of Debrecen9
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark10
Fungal Chemodiversity, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark11
New Bioactive Compounds, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark12
...and 2 moreFor a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi.
We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans.
In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
Language: | English |
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Publisher: | KeAi Publishing |
Year: | 2019 |
Pages: | 142-149 |
ISSN: | 2405805x and 20971206 |
Types: | Journal article |
DOI: | 10.1016/j.synbio.2019.08.002 |
ORCIDs: | Kiesewalter, Heiko T. , Frisvad, Jens Christian , Weber, Tilmann , Larsen, Thomas Ostenfeld , Kovács, Ákos T. and Ding, Ling |