Journal article
Kinetics of propionate conversion in anaerobic continuously stirred tank reactors
Bioenergy and Biomass, Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark1
Biosystems Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark2
Risø National Laboratory for Sustainable Energy, Technical University of Denmark3
Department of Systems Biology, Technical University of Denmark4
The kinetic parameters of anaerobic propionate degradation by biomass from 7 continuously stirred tank reactors differing in temperature, hydraulic retention time and substrate composition were investigated. In substrate-depletion experiments (batch) the maximum propionate degradation rate, A(max), and the half saturation constant, K-m, were initially estimated by applying the integrated Michaelis-Menten equation.
A(max) was in the range from 22.8 to 29.1 mu mol gVS(-1) h(-1) while K-m, was in the range from 0.46-0.95 mM. In general, A(max) gave a good reflection of the reactor performances. Secondly, the accuracy of the applied method was evaluated by use of radiotracer methodology. A(max) was found to be 14-157 lower in the substrate-depletion experiment than in the radioisotope experiment due to endogenous propionate production.
By including the endogenous propionate production, a 42-49% lower K-m, was estimated. The results demonstrate that the rate of endogenous substrate (propionate) production should be taken into account when estimating kinetic parameters in biomass from manure-based anaerobic reactors.
Language: | English |
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Year: | 2008 |
Pages: | 151-160 |
ISSN: | 1479487x and 09593330 |
Types: | Journal article |
DOI: | 10.1080/09593330802028535 |