Journal article
A new self-made digital slide scanner and microscope for imaging and quantification of fluorescent microspheres
University of Copenhagen1
Copenhagen University Hospital Herlev and Gentofte2
Department of Photonics Engineering, Technical University of Denmark3
Diode Lasers and LED Systems, Department of Photonics Engineering, Technical University of Denmark4
Department of Applied Mathematics and Computer Science, Technical University of Denmark5
Visual Computing, Department of Applied Mathematics and Computer Science, Technical University of Denmark6
Objective: A low-cost microscope slide scanner was constructed for the purpose of digital imaging of newborn piglet brain tissue and to quantify fluorescent microspheres in tissue. Methods: Using a standard digital single-lens reflex (DSLR) camera, fluorescent imaging of newborn piglet brain tissue was performed.
A computer algorithm available for download was created to detect fluorescent microspheres in the brain tissue slides and to calculate regional cerebral blood flow (rCBF). The precision of the algorithm was tested by comparing with manual counting of the fluorescent microspheres. Finally, bright-field imaging was tested by adding light diffuser film.
Results: Cost of the slide scanner was a fraction of the cost of a commercial slide scanner. The slide scanner was able to image a large number of tissue slides in a semiautomatic manner and provided a large field of view (FOV) of 101 mm2 combined with a resolution of 2.9 µm. The mean difference (SD) between manual and automatic counts was in absolute numbers 0.32 (1.5) microspheres ranging from -5 to 5 microspheres per slide.
The relative total difference between automatic and manual counts was -3.1%. Conclusions: A slide scanner was constructed and an automatic algorithm to detect fluorescent microspheres in tissue was developed and validated and showed an acceptable difference to “gold standard” manual counting. The slide scanner can be regarded as a low-cost alternative for researchers when digital slide imaging and quantification of fluorescent microspheres are needed.
Language: | English |
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Year: | 2015 |
Pages: | 33-39 |
ISSN: | 19254016 and 19254008 |
Types: | Journal article |
DOI: | 10.5430/jbgc.v5n2p33 |
ORCIDs: | Thorseth, Anders and Christensen, Anders Nymark |