Journal article
Affinity Induced Surface Functionalization of Liposomes Using Cu-Free Click Chemistry
Department of Micro- and Nanotechnology, Technical University of Denmark1
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark2
Risø National Laboratory for Sustainable Energy, Technical University of Denmark3
Department of Chemistry, Technical University of Denmark4
Functionalization of nanoparticles is a key element for improving specificity of drug delivery systems toward diseased tissue or cells. In the current study we report a highly efficient and chemoselective method for post-functionalization of liposomes with biomacromolecules, which equally well can be used for functionalization of other nanoparticles or solid surfaces.
The method exploits a synergistic effect of having both affinity and covalent anchoring tags on the surface of the liposome. This was achieved by synthesizing a peptide linker system that uses Cu-free strain-promoted click chemistry in combination with histidine affinity tags. The investigation of post-functionalization of PEGylated liposomes was performed with a cyclic RGDfE peptide.
By exploring both affinity and covalent tags a 98 ± 2.0% coupling efficiency was achieved, even a diluted system showed a coupling efficiency of 87 ± 0.2%. The reaction kinetics and overall yield were quantified by HPLC. The results presented here open new possibilities for constructing complex nanostructures and functionalized surfaces.
Language: | English |
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Publisher: | American Chemical Society (ACS) |
Year: | 2016 |
Pages: | 1673-80 |
ISSN: | 15204812 and 10431802 |
Types: | Journal article |
DOI: | 10.1021/acs.bioconjchem.6b00221 |
ORCIDs: | Bak, Martin and Andresen, Thomas Lars |