Journal article
Lab on a chip automates in vitro cell culturing
Magna Græcia University1
Smart Biosystems ApS2
Italian Institute of Technology3
Department of Chemical and Biochemical Engineering, Technical University of Denmark4
Center for Process Engineering and Technology, Department of Chemical and Biochemical Engineering, Technical University of Denmark5
A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data.
Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20μl/h. A common pH electrode is supplied by 14μl sample, which is expanded with help of DI water.
Language: | English |
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Year: | 2012 |
Pages: | 655-658 |
ISSN: | 18735568 and 01679317 |
Types: | Journal article |
DOI: | 10.1016/j.mee.2012.07.027 |
ORCIDs: | Gernaey, Krist and Krühne, Ulrich |