Journal article
FISHprep: A Novel Integrated Device for Metaphase FISH Sample Preparation
Nano-Bio Integrated Systems Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
University of Copenhagen4
Kennedy Center5
We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogenetic slide preparation, cell cultures are routinely used to process samples (for culture, arrest and fixation of cells) and/or to expand limited amount of samples (in case of prenatal diagnostics).
Arguably, this expansion and other sample pretreatments form the longest part of the entire diagnostic protocols spanning over 3–4 days. We present here a novel device with an integrated expansion chamber to culture, arrest and fix metaphase cells followed by a subsequent splashing protocol leading to ample metaphase chromosome spreads on a glass slide for metaphase FISH analysis.
The device provides an easy, disposable, low cost, integrated solution with minimal handling for metaphase FISH slide preparation.
| Language: | English |
|---|---|
| Publisher: | MDPI AG |
| Year: | 2011 |
| Pages: | 116-128 |
| ISSN: | 2072666x |
| Types: | Journal article |
| DOI: | 10.3390/mi2020116 |
| ORCIDs: | Dimaki, Maria , Svendsen, Winnie Edith , 0000-0002-6362-7973 and 0000-0001-6181-2705 |
