Journal article
Genetic Characterization of Neosartorin Biosynthesis Provides Insight into Heterodimeric Natural Product Generation
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Department of Chemistry, Technical University of Denmark2
Organic Chemistry, Department of Chemistry, Technical University of Denmark3
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Natural Product Discovery, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
A biosynthetic gene cluster of the fungal xanthone heterodimer neosartorin (1) was discovered in Aspergillus novofumigatus, and its biosynthesis was investigated by a series of gene-deletion experiments. The results indicate that the two monomeric units of 1 are synthesized by the same set of enzymes, with chrysophanol (5) as a common precursor.
Furthermore, the P450 monooxygenase NsrP for the heterodimerization was discovered, which also accepts non-native substrates to afford novel xanthone dimers.
| Language: | English |
|---|---|
| Publisher: | American Chemical Society |
| Year: | 2018 |
| Pages: | 7194-7200 |
| ISSN: | 15237052 and 15237060 |
| Types: | Journal article |
| DOI: | 10.1021/acs.orglett.8b03123 |
| ORCIDs: | Matsuda, Yudai , Gotfredsen, Charlotte Held and Larsen, Thomas Ostenfeld |
