Journal article
Escherichia coli modular coculture system for resveratrol glucosides production
Duy Tan University1
Vietnamese Academy of Science and Technology2
Thai Nguyen University3
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
iLoop, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
In bio-based fermentation, the overall bioprocess efficiency is significantly affected by the metabolic burden associated with the expression of complete biosynthetic pathway as well as precursor and cofactor generating enzymes into a single microbial cell. To attenuate such burden by compartmentalizing the enzyme expression, recently synthetic biologists have used coculture or poly-culture techniques for biomolecules synthesis.
In this paper, coculture system of two metabolically engineered Escherichia coli populations were employed which comprises upstream module expressing two enzymes converting para-coumaric acid into resveratrol and the downstream module expressing glucosyltransferase to convert the resveratrol into its glucosidated forms; polydatin and resveratroloside.
Upon optimization of the initial inoculum ratio of two E. coli populations, 92 mg resveratrol glucosides/L (236 µM) was produced i.e. achieving 84% bioconversion from 280 µM of p-coumaric acid in 60 h by 3 L fed batch fermentor. This is the report of applying coculture system to produce resveratrol glucosides by expressing the aglycone formation pathway and sugar dependent pathway into two different cells.
Language: | English |
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Publisher: | Springer Netherlands |
Year: | 2018 |
Pages: | 75 |
ISSN: | 15730972 and 09593993 |
Types: | Journal article |
DOI: | 10.1007/s11274-018-2458-z |
ORCIDs: | 0000-0001-8891-7195 and Malla, Sailesh |
3,5,4'-trihydroxystilbene-4'-O-glucoside Applied Microbiology Batch Cell Culture Techniques Biochemistry, general Biomedical and Life Sciences Bioreactors Biosynthetic Pathways Biotechnology Coculture Techniques Coumaric Acids DNA, Bacterial Environmental Engineering/Biotechnology Escherichia coli Fermentation Genetic Vectors Glucosides Glucosyltransferases Life Sciences Metabolic Engineering Microbiology Resveratrol Stilbenes Temperature polydatin