Journal article
Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism
Lactobacilli are probiotics that, among other health-promoting effects, have been ascribed immunostimulating and virus-preventive properties. Certain Lactobacillus spp. have been shown to possess strong interleukin-12 (IL-12) -inducing properties. As IL-12 production depends on the up-regulation of type I interferons (IFNs), we hypothesized that the strong IL-12-inducing capacity of Lactobacillus acidophilus NCFM in murine bone-marrow-derived dendritic cells (DCs) is caused by an up-regulation of IFN-beta, which subsequently induces IL-12 and the double-stranded RNA binding Toll-like receptor-3 (TLR-3).
The expression of the genes encoding IFN-beta, TLR-3, IL-12 and IL-10 in DCs upon stimulation with L. acidophilus NCFM was determined. Lactobacillus acidophilus NCFM induced a much stronger expression of Ifn-beta, Il-12 and Il-10 compared with the synthetic double-stranded RNA ligand Poly I:C, whereas the levels of expressed Tlr-3 were similar.
Whole genome microarray gene expression analysis revealed that other genes related to viral defence were significantly up-regulated and among the strongest induced genes in DCs stimulated with L. acidophilus NCFM. The ability to induce IFN-beta was also detected in another L. acidophilus strain (X37), but was not a property of other probiotic strains tested, i.e.
Bifidobacterium bifidum Z9 and Escherichia coli Nissle 1917. The IFN-beta expression was markedly reduced in TLR-2(-/-) DCs, dependent on endocytosis, and the major cause of the induction of Il-12 and Tlr-3 in DCs stimulated with L. acidophilus NCFM. Collectively, our results reveal that certain lactobacilli trigger the expression of viral defence genes in DCs in a TLR-2 manner dependent on IFN-beta.
Language: | English |
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Publisher: | Blackwell Science Inc |
Year: | 2010 |
Pages: | 268-281 |
ISSN: | 13652567 , 00192805 and 09534954 |
Types: | Journal article |
DOI: | 10.1111/j.1365-2567.2010.03301.x |
ORCIDs: | Rasmussen, Simon , Jarmer, Hanne Østergaard and 0000-0002-2555-5948 |
Animals Antibodies Clathrin Cytokines Dendritic Cells Endocytosis Gene Expression Gene Expression Profiling Gene Expression Regulation Immunity, Innate Interferon-beta Interleukin-12 Lactobacillus acidophilus Mice Mice, Inbred C57BL Mice, Knockout Oligonucleotide Array Sequence Analysis Poly I-C Probiotics TLR3 protein, mouse Tlr2 protein, mouse Toll-Like Receptor 2 Toll-Like Receptor 3 Toll-like receptor-2 Up-Regulation dendritic cells gene regulation innate immune response virus