Journal article
Injection molded chips with integrated conducting polymer electrodes for electroporation of cells
Polymer Microsystems for Electrophysiology Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Polymer Microsystems for Cell Processing Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Department of Photonics Engineering, Technical University of Denmark5
Optical Microsensors and Micromaterials, Department of Photonics Engineering, Technical University of Denmark6
Amphiphilic polymers in biological sensing Group, Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark7
Self-organizing materials for nanotechnology Section, Department of Micro- and Nanotechnology, Technical University of Denmark8
We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented.
The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz, +/- 50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
| Language: | English |
|---|---|
| Year: | 2010 |
| Pages: | 055010 |
| ISSN: | 13616439 and 09601317 |
| Types: | Journal article |
| DOI: | 10.1088/0960-1317/20/5/055010 |
| ORCIDs: | Larsen, Niels Bent and Taboryski, Rafael Jozef |
