Journal article
FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images
German Electron Synchrotron1
Department of Applied Mathematics and Computer Science, Technical University of Denmark2
Department of Physics, Technical University of Denmark3
Neutrons and X-rays for Materials Physics, Department of Physics, Technical University of Denmark4
Computational Atomic-scale Materials Design, Department of Physics, Technical University of Denmark5
A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues-Frank space, in which all crystal systems can be represented without singularities.
The new algorithm is shown to be capable of indexing more than ten crystals per image in simulations of cubic, tetragonal and monoclinic crystal diffraction patterns. It is also used to index an experimental serial crystallography dataset from lysozyme microcrystals. The increased number of indexed crystals is shown to result in a better signal-to-noise ratio, and fewer images are needed to achieve the same data quality as when indexing one crystal per image.
The relative orientations between the multiple crystals indexed in an image show a slight tendency of the lysozme microcrystals to adhere on (110) facets.
Language: | English |
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Publisher: | International Union of Crystallography |
Year: | 2017 |
Pages: | 1075-1083 |
ISSN: | 16005767 and 00218898 |
Types: | Journal article |
DOI: | 10.1107/S1600576717007506 |
ORCIDs: | 0000-0002-7701-4369 , Larsen, Peter Mahler and Schmidt, Søren |