Journal article
Multiple determinants in the coding region of Pea seed-borne mosaic virus P3 are involved in virulence against sbm-2 resistance
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Technical University of Denmark4
Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark5
National Food Institute, Technical University of Denmark6
National Veterinary Institute7
Viral determinants for overcoming Pisum sativum recessive resistance, sbm-2, against the potyvirus Pea seed-borne mosaic virus (PSbMV) were identified in the region encoding the N-terminal part of the P3 protein. Codons conserved between sbm-2 virulent isolates in this region: Q21, K30 and H122 were found to specifically impair sbm-2 virulence when mutated in selected genetic backgrounds.
The corresponding amino acids, Gln21 and Lys30, are neighbored by P3 residues strongly conserved among potyviruses and His122 is conserved particularly in potyviral species infecting legumes. The strongest selective inhibition of sbm-2 virulence, however, was observed by elimination of isolate specific length polymorphisms also located in the N-terminal part of the P3 protein.
Length variation in N-terminal P3 is common between potyviral species. However, intra-species length polymorphism in this region was found only among PSbMV isolates. Our findings comply with a model for PSbMV pathotypes having evolved by a diversification of the P3 protein likely to extend to the level of function.
Language: | English |
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Year: | 2006 |
Pages: | 52-61 |
ISSN: | 1089862x , 00426822 and 10960341 |
Types: | Journal article |
DOI: | 10.1016/j.virol.2006.07.016 |
ORCIDs: | 0000-0001-9591-3897 and Hjulsager, Charlotte Kristiane |
Amino Acid Sequence Amino Acid Substitution Codon Conserved Sequence Immunity, Innate Molecular Sequence Data Mutation P3 protein Pathotype Pisum sativum Plant Diseases Polymorphism, Genetic Potyviridae Potyvirus Recessive resistance Sequence Deletion Sequence Homology, Amino Acid Sequence alignment Viral Proteins Virulence