Journal article
Comparative chemistry of Aspergillus oryzae (RIB40) and A. flavus (NRRL 3357)
Department of Systems Biology, Technical University of Denmark1
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark2
Organic Chemistry, Department of Chemistry, Technical University of Denmark3
Department of Chemistry, Technical University of Denmark4
Aspergillus oryzae and A. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. Bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production.
In this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of A. oryzae (RIB40) and A. flavus (NRRL 3357). Surprisingly, the overall chemical profiles of the two strains were mostly very different across 15 growth conditions. Contrary to previous studies we found the aflatrem precursor 13-desoxypaxilline to be a major metabolite from A. oryzae under certain growth conditions.
For the first time, we additionally report A. oryzae to produce parasiticolide A and two new analogues hereof, along with four new alkaloids related to the A. flavus metabolites ditryptophenalines and miyakamides. Generally the secondary metabolite capability of A. oryzae presents several novel end products likely to result from the domestication process from A. flavus.
| Language: | English |
|---|---|
| Publisher: | MDPI |
| Year: | 2012 |
| Pages: | 39-56 |
| ISSN: | 22181989 |
| Types: | Journal article |
| DOI: | 10.3390/metabo2010039 |
| ORCIDs: | Frisvad, Jens Christian , Gotfredsen, Charlotte Held and Larsen, Thomas Ostenfeld |
(NRRL 3357) (RIB40) Aspergillus flavus Aspergillus oryzae ditryptoleucine oryzamide parasiticolide
