Journal article
Alkalilactibacillus ikkensis, gen. nov., sp. nov., a novel enzyme-producing bacterium from a cold and alkaline environment in Greenland
National Food Institute, Technical University of Denmark1
Division of Epidemiology and Microbial Genomics, National Food Institute, Technical University of Denmark2
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark3
University of Copenhagen4
Aarhus University5
Three novel Gram-positive, endospore-forming bacteria were isolated from a cold and alkaline environment. Phylogenetic analysis showed that the strains were almost identical, and that they were related to Natronobacillus azotifigens 24KS-1(T) (95.8% identity), Paraliobacillus quinghaiensis YIM-C158(T) (95.1%), Paraliobacillus ryukyuensis O15-7(T) (94.5%), and Halolactibacillus miurensis M23-1(T) (93.9%).
The isolates produced amylase, α-galactosidase, β-galactosidase, and β-glucuronidase, and showed optimal growth at pH 10, at 20°C, and at 2-8% (w/v) NaCl. Major fatty acids were C(14:0) (10.6-11.6%), anteiso-C(15:0) (25.7-32.7%), C(16:1) ω11c (12.2-16.0%), and C(16:0) (14.0-20.4%).
The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and meso-diaminopimelic acid was found in the cell-wall peptidoglycan. The G+C content was 38.4%. DNA-DNA hybridization between strain GCM68(T) and H. miurensis M23-1(T) was 32.4%, while hybridization to N. azotifigens 24KS-1(T), Amphibacillus tropicus Z-7792(T), and Paraliobacillus ryukyuensis O15-7(T) was below 30%.
The phylogenetic analysis and G+C content place strain GCM68(T) in relation to species belonging to Bacillus rRNA group 1, but phylogenetic and physiologic data combined with chemotaxonomic analyses support our proposal for a new genus, Alkalilactibacillus, gen. nov., with the novel species Alkalilactibacillus ikkensis, sp. nov. (type strain is GCM68(T) = DSM 19937 = LMG 24405).
Language: | English |
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Publisher: | Springer Japan |
Year: | 2012 |
Pages: | 297-305 |
Journal subtitle: | Microbial Life Under Extreme Conditions |
ISSN: | 14334909 and 14310651 |
Types: | Journal article |
DOI: | 10.1007/s00792-012-0430-7 |
ORCIDs: | 0000-0002-9925-067X and 0000-0002-2796-7137 |