Journal article
Effects of antibiotics on quorum sensing in pseudomonas aeruginosa
Department of Systems Biology, Technical University of Denmark1
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark2
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark3
Copenhagen University Hospital Herlev and Gentofte4
Chr. Hansen AS5
During infection, Pseudomonas aeruginosa employs bacterial communication (quorum sensing [QS]) to coordinate the expression of tissue-damaging factors. QS-controlled gene expression plays a pivotal role in the virulence of P. aeruginosa, and QS-deficient mutants cause less severe infections in animal infection models.
Treatment of cystic fibrosis (CF) patients chronically infected with P. aeruginosa with the macrolide antibiotic azithromycin (AZM) has been demonstrated to improve the clinical outcome. Several studies indicate that AZM may accomplish its beneficial action in CF patients by impeding QS, thereby reducing the pathogenicity of P. aeruginosa.
This led us to investigate whether QS inhibition is a common feature of antibiotics. We present the results of a screening of 12 antibiotics for their QS-inhibitory activities using a previously described QS inhibitor selector 1 strain. Three of the antibiotics tested, AZM, ceftazidime (CFT), and ciprofloxacin (CPR), were very active in the assay and were further examined for their effects on QS-regulated virulence factor production in P. aeruginosa.
The effects of the three antibiotics administered at subinhibitory concentrations were investigated by use of DNA microarrays. Consistent results from the virulence factor assays, reverse transcription-PCR, and the DNA microarrays support the finding that AZM, CFT, and CPR decrease the expression of a range of QS-regulated virulence factors.
The data suggest that the underlying mechanism may be mediated by changes in membrane permeability, thereby influencing the flux of N-3-oxo-dodecanoyl-L-homoserine lactone.
Language: | English |
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Publisher: | American Society for Microbiology (ASM) |
Year: | 2008 |
Pages: | 3648-3663 |
ISSN: | 10986596 , 00664804 and 10706283 |
Types: | Journal article |
DOI: | 10.1128/AAC.01230-07 |
ORCIDs: | 0000-0002-8003-7414 , 0000-0002-9751-474X , 0000-0002-1347-725X and 0000-0001-5004-8609 |
Anti-Bacterial Agents Azithromycin Base Sequence Ceftazidime Ciprofloxacin DNA Primers DNA, Bacterial Gene Expression Genes, Bacterial Humans Oligonucleotide Array Sequence Analysis Opportunistic Infections Polymerase Chain Reaction Pseudomonas Infections Pseudomonas aeruginosa Quorum Sensing Virulence