Journal article
Fate and survival of Campylobacter coli in swine manure at various temperatures
Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark1
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Department of Micro- and Nanotechnology, Technical University of Denmark4
Campylobacter coli is the most common Campylobacter species found in pig(95%),but the ability of this bacterium to survive in swine manure as well as the potential for causing human illness are poorly understood. We present here laboratory-scale experiments to investigate the effect of temperature on the survival of C.coli in spiked swine manure samples at temperatures from 4 to 52˚C.
The survival of C.coli during storage for 30 days was studied by three different methods: bacterial culture(plate counting),DNA qPCR, and mRNART-qPCR.The results indicate that C.coli could survive in swine manure up to 24 days at 4˚C. At higher temperatures,this bacterium survived only 7 days(15˚C)or 6 days(22˚C)of storage.
The survival of C.coli was extremely short(few hours)in samples incubated at 42 and 52 ˚C. The results from the RT-qPCRmethodwereconsistentwiththedatafromthebacterialculturemethod, indicating that it detected only viable C.colicells,thus eliminating false-positive resulting from DNA from dead C.coli cells.
Language: | English |
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Publisher: | Frontiers Research Foundation |
Year: | 2011 |
Pages: | 262 |
ISSN: | 1664302x , 10959998 and 07400020 |
Types: | Journal article |
DOI: | 10.3389/fmicb.2011.00262 |
ORCIDs: | Wolff, Anders and Bang, Dang Duong |