Journal article
Integrated optical measurement system for fluorescence spectroscopy in microfluidic channels
Department of Photonics Engineering, Technical University of Denmark1
ChemLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark3
Department of Micro- and Nanotechnology, Technical University of Denmark4
A transportable miniaturized fiber-pigtailed measurement system is presented which allows quantitative fluorescence detection in microliquid handling systems. The microliquid handling chips are made in silica on silicon technology and the optical functionality is monolithically integrated with the microfluidic channel system.
This results in inherent stability and photolithographic alignment precision. Permanently attached optical fibers provide a rugged connection to the light source, detection, and data processing unit, which potentially allows field use of such systems. Fluorescence measurements with two dyes, fluorescein, and Bodipy 650/665 X, showed good linear behavior over a wide range of concentrations.
Minimally detected concentrations were 250 pM for fluorescein and 100 nM for Bodipy.
| Language: | English |
|---|---|
| Publisher: | American Institute of Physics |
| Year: | 2001 |
| Pages: | 229-233 |
| ISSN: | 10897623 and 00346748 |
| Types: | Journal article |
| DOI: | 10.1063/1.1326929 |
| ORCIDs: | Hübner, Jörg and Jørgensen, Anders Michael |
