Journal article
A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression
Department of Veterinary Pathobiology, University of Copenhagen, Ridebanevej, Frederiksberg, Denmark.1
A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein.
Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue.
Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.
Language: | English |
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Year: | 2008 |
Pages: | 64-70 |
ISSN: | 18727980 and 03043835 |
Types: | Journal article |
DOI: | 10.1016/j.canlet.2007.11.030 |
ORCIDs: | Birkenkamp-Demtroder, Karin |