Journal article
Tandem Repeat Analysis for Surveillance of Human Salmonella Typhimurium Infections
In Denmark, as part of the national laboratory-based surveillance system of human enteric infections, all Salmonella enterica serotype Typhimurium isolates are currently subtyped by using phage typing, antimicrobial resistance profiles, and pulsed-field gel electrophoresis (PFGE). We evaluated the value of real-time typing that uses multiple-locus variable-number tandem-repeats analysis (MLVA) of S.
Typhimurium to detect possible outbreaks. Because only a few subtypes identified by PFGE and phage typing account for most infections, we included MLVA typing in the routine surveillance in a 2-year period beginning December 2003. The 1,019 typed isolates were separated into 148 PFGE types and 373 MLVA types.
Several possible outbreaks were detected and confirmed. MLVA was particularly valuable for discriminating within the most common phage types. MLVA was superior to PFGE for both surveillance and outbreak investigations of S. Typhimurium.
| Language: | English |
|---|---|
| Publisher: | Centers for Disease Control and Prevention |
| Year: | 2007 |
| Pages: | 388-395 |
| ISSN: | 10806040 and 10806059 |
| Types: | Journal article |
| DOI: | 10.3201/eid1303.060460 |
Anti-Bacterial Agents Bacteriophage Typing Denmark Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Humans Infectious and parasitic diseases MLVA Medicine Minisatellite Repeats Molecular Epidemiology PFGE R RC109-216 Salmonella Salmonella Infections Salmonella typhimurium Sentinel Surveillance Species Specificity Tandem Repeat Sequences Typhimurium VNTR outbreak phagetyping research surveillance
